منابع مشابه
Ascorbate oxidase.
A new procedure has been described for the isolation and purification of ascorbate oxidase from green zucchini squash (Cucurbita pepo meduttosa). The new purification method achieves a more than ZOO-fold purification with about a 14% recovery of the total activity present in the crude juice. The purified enzyme preparations have been found to be homogeneous by the criteria of ultracentrifugal a...
متن کاملElectrochemical assay for brain ascorbate with ascorbate oxidase.
cision is not better than 2% of the maximum concentration of each concentration range. This means that an error of 0.02 mg/L S in the 0-1 mg/L S range corresponds to an error of 1 mg/L S in the 0-50 mg/L S range. For optimum precision, the response of the analyzer should be kept large by selecting the syringe size according to the sulfur content of the analyte and maintaining a reasonably rapid...
متن کاملThe function of ascorbate oxidase in tobacco.
The function of the apoplastic enzyme ascorbate oxidase (AO) was investigated in tobacco (Nicotiana tabacum). The abundance of AO mRNA was up-regulated by light. Cytosolic ascorbate peroxidase (APX1) transcripts were also highest in the light. In contrast, L-galactono-gamma-lactone dehydrogenase, stromal APX, and thylakoid APX transcripts remained constant over the day/night cycle. Salicylic ac...
متن کاملAscorbic Acid Determination by an Amperometric Ascorbate Oxidase-based Biosensor
The aim of this work is to develop a method for ascorbic acid determination in some fruit juices, by using an ascorbate oxidase-based amperometric enzyme electrode. The enzyme was immobilized on a nylon (Biodyne A) membrane by glutaraldehyde linking. The enzyme-membrane was consecutively attached to the polyethylene membrane of a Clark oxygen electrode, which functioned as transducer. The analy...
متن کاملAscorbate oxidase and its possible involvement in cancer.
The electron spin resonance (ESR) spectrum of erythrocytes of a health male volunteer exhibited 2 min after intravenous administration of 1g of ascorbic acid a considerable increase in spin concentration and an new signal at about g-2.005 which we previously had found to correlate to the semidehydroascorbate (SDA) radical and which is not identical with the O2 radical. Moreover, the vitamin C c...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43395-4